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Several biological and chemical factors determine how long a peptide remains active or detectable in the body

Ghanem and co-workers reported similar observations of increased antioxidant activity and improved blastocyst quality by way of higher embryo development rate and fewer number of apoptotic cells after supplementing LC (1.5 mM, equivalent to 0.3 mg/mL) in culture media of bovine blastocyst during the pre-implantation stage [67]

After 20 min incubation at 4 C, cells were centrifuged at 1000 rpm for 5 min (Eppendor 5910R, Hambourg, Germany)

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